Karyotype Analysis of Rheum officinale Chromosome

Karyotype analysis of Rheum palmatum.

Rheum palmatum, one of the source plants of the traditional Chinese medicine rhubarb, is anendemic and endangered species. To our knowledge, this is the first report on the chromosome number and karyotype of this species. Sectioning combined with micrography was used to analyze the karyotype. The following results were obtained: R. palmatum had a stable chromosome number 2n = 22; the basic numb...

Karyotype Analysis and Chromosome Banding

Nearly all methods of chromosome banding rely on harvesting chromosomes in mitosis. This is usually achieved by treating cells with tubulin inhibitors, such as colchicine or demecolcine (colcemid), that depolymerize the mitotic spindle and so arrest the cell at this stage. Excessively long incubations with Colcemid result in overcondensed chromosomes that band poorly and moreover some cell type...

Optimization of chloroplast microsatellite PCR conditions and primer screening for endangered Rheum officinale, Rheum palmatum, and Rheum tanguticum.

Chloroplast microsatellite primers were developed in order to provide more population genetic information of endangered Rheum officinale, R. palmatum, and R. tanguticum for conservation. The dried roots and rhizomes of these plants are important in traditional Chinese medicine. The results showed that the optimum concentrations of Mg(2+), Taq DNA polymerase, dNTPs, template DNA, and primers in ...

Karyotype analysis and new chromosome number reports for nine Vicia species in Iran

This study concerns diploid chromosome numbers and karyotype analysis of nine species belonging to four sections (Vicilla, Cracca, Variegata and Anatropostylia) of the subgenus Vicilla of the genus Vicia (Fabaceae). The chromosome numbers of Vicia alpestris subsp. hypoleuca, V. aucheri, V. ciceroideae, V. gariensis, V. iranica, V. koeieana, V. rechingeri, V. sojakii and V. venulosa are reported...

Karyotype and Sex-Chromosome Polymorphism in Nesokia indica, from Iran